Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• ; nanoarchitectonics; information visualization; sandwich-type immunosensors; screen-printed electrodes; Introduction The prostate-specific antigen (PSA) used in clinical diagnosis is present in normal prostatic secretions, but its concentration is often elevated in prostate cancer patients. In spite of its lack of

• immobilized on the electrode surface. Also, the monoclonal antibody provides high specificity to a single epitope, which is reflected in a low cross-reactivity. A comparison of various sandwich-type immunosensors and immunoassays for detection of PSA in the literature is presented in Table 1. Repeatability is

• incubated with 5 μL of bovine serum albumin (BSA) (2% w/w) diluted in phosphate-buffered saline (PBS), to avoid non-specific binding. Preparation of the bioconjugate complex of Ab2 and HRP (Ab2-MNP-HRP). Illustration of the PSA capturing step. Comparison of various sandwich-type immunosensors and